Part:BBa_K5090008:Design

Codon-Optimized GST-Argonaute2 Device

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 3180
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2031
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1103
  Illegal SapI.rc site found at 132
  

Design Notes

The main basic part for this composite part is codon-optimized for expression in E. coli, and the promoter, RBS, and terminator are all chosen to be optimal for E. coli/S30 cell-free system expression.

Source

This part is composed of several sub-parts, listed in the sequence.